Akwai nau'ikan rini iri-iri da ake amfani da su wajen yin bioslicing, kowannensu yana da halaye daban-daban da kuma nau'ikan aikace-aikacensa. Ga wasu rini na bioslicing da ake amfani da su akai-akai da kuma taƙaitaccen gabatarwarsu:
Da farko, rini na halitta
Hematoxylin: Wannan wani nau'in fenti ne da aka samo daga busasshen rassan hematoxylum na Kudancin Amurka (legume na wurare masu zafi) ta hanyar jiƙa shi a cikin ether. Ba za a iya rina Hematoxylin kai tsaye ba, kuma yana buƙatar a yi masa oxidizing don ya zama oxyhematoxylin (wanda kuma ake kira hematoxylin) kafin a yi amfani da shi. Abu ne mai kyau don yin fenti a tsakiya kuma yana iya bambance tsari daban-daban a cikin tantanin halitta zuwa launuka daban-daban.
Carmine: Carmine, wanda aka fi sani da carmine ko carmine, ana yin sa ne daga ƙwaro na cochineal na mata masu zafi, waɗanda aka busar sannan aka niƙa su zuwa foda, aka cire su da jajayen kwari, sannan aka yi musu magani da alum don yin sa. Carmagenta kuma rini ne mai kyau ga ƙwayoyin halittar, kuma samfurin da aka rina ba shi da sauƙin lalacewa, musamman ga rini ga ƙananan kayayyaki gaba ɗaya.
Na biyu, rini na wucin gadi
Fuchsin mai sinadarin acid: Fuchsin mai sinadarin acid ne, foda ja, yana narkewa a cikin ruwa, yana narkewa kaɗan a cikin barasa. Yana da kyau wajen tsaftace tantanin halitta, ana amfani da shi sosai wajen shirya dabbobi, wajen shirya tsirrai don fata, ɓangaren litattafan almara da sauran ƙwayoyin halitta na parenchyma da bangon cellulose.
Ja na Congo: Ja na Congo rini ne mai tsami, a cikin siffar foda ja na jujube, yana narkewa a cikin ruwa da barasa, shuɗi a cikin acid. Sau da yawa ana amfani da shi wajen samar da tsire-tsire a matsayin layin hematoxylin ko wasu rini na tantanin halitta, kuma ana iya amfani da shi don yin tabo a cikin cytoplasm da gatari na jijiyoyi.
Kore mai ƙarfi: Kore mai ƙarfi rini ne mai tsami, yana narkewa a cikin ruwa da barasa. Wani nau'in rini ne na ƙwayoyin cellulose da ke ɗauke da plasma, wanda ake amfani da shi sosai a cikin ƙwayoyin rini da kyallen tsirrai.
Sudan ta Uku: Sudan ta Uku rini ne mai rauni na acid, foda ja, wanda ke narkewa a cikin kitse da barasa. Tabon kitse ne wanda galibi ana amfani da shi don nuna yawan kitsen da ke cikin kyallen takarda.
Eosin: Akwai nau'ikan eosin da yawa, kuma eosin Y da ake amfani da shi akai-akai rini ne mai acidic, wanda yake ja da ƙananan lu'ulu'u masu shuɗi ko foda mai launin ruwan kasa. Ana amfani da Eosin sosai wajen shirya dabbobi, rini ne mai kyau na cytoplasmic, kuma galibi ana amfani da shi azaman rini mai haɗa hematoxylin.
Fuchsin na asali: Fuchsin na asali rini ne na alkaline, wanda ake amfani da shi sosai wajen samar da halittu kuma ana iya amfani da shi don yin tabo ga zaruruwan collagen da zaruruwan roba.
Lu'ulu'u mai launin shuɗi: Lu'ulu'u mai launin shuɗi wani nau'in alkaline ne, wanda ake amfani da shi sosai a fannin cytology, histology da bacteriology, tabo ne mai kyau, wanda galibi ana amfani da shi don tabon nukiliya.
Violet na Gentian: Violet na Gentian cakuda ne na rini na alkaline, galibi cakuda violet na crystal da methyl violet, waɗanda za a iya amfani da su a musanya da violet na crystal idan ya cancanta.
Waɗannan rini suna taka muhimmiyar rawa wajen yanke ƙwayoyin halitta, kuma ta hanyar hanyoyi daban-daban na yin tabo da haɗuwa, suna iya nuna tsarin halittar ƙwayoyin halitta da kyallen halitta a sarari, suna ba da muhimmiyar goyon bayan fasaha ga binciken halittu da na likitanci.
Lokacin Saƙo: Agusta-08-2024