Masu kera sassan halittu: Yadda ake bambance tsakanin manne da lodawa

A fannin sashen halittu, smear da installing ra'ayoyi ne daban-daban guda biyu, kuma bambancinsu ya fi yawa ne a kan yadda ake sarrafa samfurin da kuma yadda aka shirya sashen.

Smear: Smear yana nufin hanyar shiryawa ta shafa samfurin kai tsaye a kan zamiya. Yawanci ana shafa smear a kan samfuran ruwa ko samfuran ƙwayoyin halitta, kamar jini, ruwan cerebrospinal, fitsari, da sauransu. A lokacin shirya smear, ana cire samfurin a shafa kai tsaye a kan zamiya, wanda daga nan aka rufe shi da wani zamiya don samar da takardar bugawa, wanda aka yi masa fenti ta hanyar takamaiman hanyar tabo. Yawanci ana amfani da smear don nazarin ƙwayoyin halitta don duba yanayin ƙwayoyin halitta da tsarinsu a cikin samfurin.

Lodawa: Lodawa yana nufin hanyar shiryawa ta gyara samfurin nama, yanke shi zuwa yanka siriri da microtome, sannan a haɗa waɗannan yanka zuwa zamiya. Yawanci, hawa ya dace da samfuran nama masu ƙarfi, kamar yanka nama, tubalan ƙwayoyin halitta, da sauransu. A lokacin shirya hawa, ana fara gyara samfurin, a bushe shi, a tsoma shi a cikin kakin zuma, da sauransu, sannan a yanka shi zuwa yanka siriri ta hanyar microtome, sannan a haɗa waɗannan yanka a zamiya don rini. Yawanci ana amfani da hoton don binciken histological don lura da tsarin nama da canje-canjen cututtuka.

Saboda haka, mabuɗin bambance tsakanin smear da loading yana cikin tsarin sarrafa samfurin da shirya shi. Smear shine hanyar shiryawa ta shafa samfurin kai tsaye akan zamiya, wanda ya dace da samfuran ruwa ko samfuran tantanin halitta; Lodawa ita ce hanyar shiryawa ta yanke samfurin nama mai ƙarfi zuwa yanka siriri sannan a haɗa shi da zamiya, wanda ya dace da samfuran nama mai ƙarfi.

Alamu Masu Alaƙa: Biopexy, Masu kera Biopexy, Biopexy, Masu kera samfurin samfuri,