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Kafa kayan dabbobin dabbobi na canji na modic (MC) muhimmin tushe ne ga nazarin MC. An raba manyan zomaye na yau da kullun da hamsin da aka kasu kashi biyu, rukunin tsoka na tsoka (min rukuni) da rukunin nucleus). A cikin rukunin npe, an fallasa diskokin masu linzami da cututtukan ƙwayar cuta na ciki da kuma allura don tsara jiki na l5 vertebral a kusa da farantin ƙarshen. NP da aka fitar daga L1 / 2 Invertebral Disc Ta hanyar sirinji da allura a ciki. Hako wani rami a cikin kashi na subchondral. Hanyoyin tiyata da hanyoyin hakowa a cikin rukunin tsoka da ƙungiyar-shagon Sham sun yi daidai da waɗanda ke cikin rukunin NP. A cikin me rukuni, an sanya yanki na tsoka a cikin rami, yayin da a cikin ƙungiyar-shago, ba abin da aka sanya shi cikin rami. Bayan aikin, MRI bincika an yi gwajin kwayoyin halitta. Alamar ta canza, amma babu wani canji na musamman canjin kungiyar da ni kungiyar. Kulawar tarihi ya nuna cewa an cika yaduwar ƙwayar nama a cikin shafin da ba shi da ƙarfi, da kuma furcin Il-4, IF-17 da IFN-γ ya ƙaru a cikin NPE kungiyar. Contlantation na NP cikin kasusuwa na subchondrong na iya samar da tsarin dabba na MC.
Canje-canje na Modic (MC) sune raunuka na tsallakewa da kuma alamar ƙwararren ƙwararraki a bayyane akan tunanin magnetic mai ɗaukar hoto (MRI). Suna da kowa da kowa a cikin mutane tare da alamun da ke tattare da alamu1. Yawancin karatu sun jaddada mahimmancin kungiyar MC saboda kungiyar ta tare da karancin baya (LBP) 2,3. De Roos et al.4 da modic et al.5 da farko da aka fara bayyana nau'ikan sigogi guda uku daban-daban a cikin bargo na pertebral kashi barna. Nau'in Modic i canje-canje a kan t1-walwed (T1w) a kan T2-izuwa (T2w). Wannan Lesion ta bayyana yassari da tsallakewa da kuma granizaren jijiyoyin jini a cikin rumbun. Modic nau'in canje-canje na II yana nuna alamar alama a ɓangarorin T1w da T2w. A cikin wannan nau'in rauni, ana iya samun halakarwa, da kuma ƙididdiga mai sihiri na ƙashi na ƙashi. Canje-canje na III na III na III suna nuna ƙarancin sigina a cikin jerin T1w da T2w jerin. An lura da raunukan ƙwayoyin cuta mai dacewa da ƙarshen ƙarshen sclerotics6. MC an dauki shi wata cuta ta circological na kashin baya kuma tana da alaƙa da cututtukan da degersative da yawa na kashin baya7,8,9.
La'akari da bayanan da ake samu, nazarin da yawa sun samar da cikakkiyar fahimta a cikin iyoron iyoron da ta MC. Albert et al. An ba da shawarar cewa MC na iya haifar da diski na Herniation8. Hu et al. danganta MC zuwa mummunan disc degeneration10. KROC da aka gabatar da manufar "diski na ciki," wanda ke bayyana raunin diski na iya haifar da microtars a cikin ƙarshen. Bayan craft samarwa, halakarwa ta tsallaka ta hanyar nucleus pulposus (NP) na iya haifar da amsawar autoimmin, wanda ke haifar da haɓakar MC11. Mah et al. Ya raba irin wannan kallo kuma ya ruwaito cewa tsarin kai tsaye na NP-Indudared yana taka muhimmiyar rawa a cikin pathogeneesis na MC12.
Sellar jikin rigakafi, musamman CD4 + T Taimaka Lymphococytes, kunna mahimmancin rawa a cikin pathogenesis na autoimmunity13. Cytetungiyar ta gano Th17 ta samar da Cytokine il-17, yana inganta faɗakarwar chatterfine il-17, yana inganta faɗakarwar chemoke, kuma yana ƙarfafa Tels a gabobin da suka lalace don samar da IFN-γ14. Sells Th2 kuma suna taka rawa na musamman a cikin cututtukan cututtukan ciki. Bayanin IL-4 azaman sel na wakili na iya haifar da sakamako mai ƙarfi na rigakafi.
Kodayake an gudanar da karatun asibiti da yawa a kan MC16,28,28,21,21,21,21,24,21, har yanzu akwai rashin dace da halayen MC wanda akai-akai yakan faru a cikin mutane kuma yana iya zama An yi amfani da shi don bincika Etiology ko sababbin jiyya kamar niyya. Zuwa yau, kawai 'yan nau'ikan dabbobi na MC an ruwaito don yin nazarin ainihin hanyoyin ƙwayoyin cuta.
Dangane da Albert da Albert da Mobert da Ma, wannan binciken ya kafa mai sauqi mai sauƙi da kuma samfurin Rabbit MC na Autotransplants na Autotbernsplanting Etant farantin. Sauran manufofin za su lura da sifofin hadidamical na ƙirar dabbobi kuma suna kimanta takamaiman hanyoyin NP a cikin ci gaban MC. Har zuwa wannan, muna amfani da dabaru kamar dabarun kwayoyin cuta, MRI, da kuma karatun tarihi, da nazarin masana tarihi don nazarin ci gaban MC.
Zomaye biyu sun mutu sakamakon zub da jini a lokacin tiyata, zomaye hudu sun mutu a lokacin da ba a shafa ba. Sauran zomaye 48 sun tsira kuma sun nuna alamun halaye ko alamun neurological bayan tiyata.
MRI yana nuna cewa siginar ƙarfi na ƙwayar da aka saka a cikin ramuka daban daban. An bayyana siginar da ke tattare da kungiyar ta L5 a hankali a cikin 'yan makonnin da aka samu a 12, da sashe na T1W sun nuna karancin siginar da lowal) (Fig. 1C), yayin da MRI. na sauran rukunoni biyu na abubuwan da aka saka sun kasance cikin tsayayye a daidai lokacin (Fig. 1A, B).
(A) Wakilin Seetarewa na Murnar zomo lumbar kashin baya a maki 3 maki. Ba a samo alamun alamun alama a cikin hotunan ƙungiyar Sham-aiki ba. (B) Halayen siginar jikin a cikina a cikin kungiyar da ke cikin rukunin Sham, kuma babu wani muhimmin canji a shafin farko. (C) A cikin rukunin npe, ƙarancin siginar a bayyane yake a cikin T1w jerin, kuma siginar gauraye da ƙarancin sigina suna bayyane a cikin T2w jerin. Daga tsawon makonni 12 zuwa lokacin sati 20, alamu mai girma da ke dauke da karancin sigina a cikin jerin jerin T2w.
Za'a iya ganin hyperpasin kashi a cikin shafin da ke tattare da gyaran jikin a cikin rukunin kungiyoyi 12 zuwa 20 (Babu wani canji mai mahimmanci a cikin upered vertebral gawarwakin; Groupungiyar Sham da ni Group (Fig. 2C) 2a, b).
(A) saman jikin vertebal a cikin yankin da aka keɓe yana da laushi, rami warkarwa yana da laushi, rami yana da kyau, kuma babu wani hyperplassia a cikin jikin vertebal. (B) Siffar gidan da aka ɗora a cikin Miya da ke kama da cewa a cikin ayyukan aiki, kuma babu wani canji a bayyanar da shafin da aka shirya akan lokaci. (C) Hyperplassia kashi ya faru a wurin da aka sanya a cikin rukunin npe. Hyperplassia na kashi ya karu da sauri kuma har ya ƙare ta hanyar diski na tsakani zuwa jikin gidan yanar gizo na ɗan uwa da jikin vertobal.
Binciken masana tarihi yana samar da cikakken bayani game da samuwar kashi. Hoto na 3 yana nuna hotunan sassan abubuwan da ke cikin ɓoye tare da H & E. A cikin kungiyar-Sham. An shirya ChundroCecCacc da aka samu sosai kuma ba a gano yunkuri na kwayar halitta (Fig.). Halin da ake ciki a cikin MEO gungun ya yi kama da cewa a cikin ƙungiyar-Sham-Gudanar da (Fig. 3b). Koyaya, a cikin NPE kungiyar, adadi mai yawa na Chondrocytes da yaduwar sel kamar an lura da sel kamar yadda aka lura da shi a cikin shafin da ke da (Fig. 3C);
(A) Za'a iya ganin Trabecula a kusa da farantin ƙarshen, ana shirya su da kyau tare da girman ƙwayar sel stedured da siffar su ba. (B) Halin shafin yanar gizon da ke tattare da shi a cikin MEO gungun ya yi kama da na ƙungiyar Sham. Trabeculaile da Chundrocacytes ana iya ganinsu, amma babu wani yaduwar yaduwar a shafin da ke tattare da shi (sau 40). (B) Ana iya ganin cewa Cheddoccytes da NP-kamar sel - kamar sel prundrafate muhimmanci, da kuma sifar da girman chondroccytes (40 sau).
Bayanin Mrna, (IL-4) MRNA, CIGABA 17 (IL-17) MPNA, da internon γ) an lura da Magna a cikin Npe da ni. Lokacin da aka kwatanta matakan da aka yi na kwayoyin halittar Il-4, IMA-17, kuma Ilyn-γ. 4) (P <0.05). Idan aka kwatanta da ƙungiyar Sham, matakan bayyanar Il-4, IMA-17, kuma Ilyn-γ Arction ya karu da canjin ƙididdiga (p> 0.05).
Bayanin Mrna na IL-4, IF-17 da IFN-γ a cikin NPE kungiyar da ya nuna matukar damuwa fiye da wadanda ke cikin aikin aikin Sham da ni (P <.05).
Ya bambanta, matakin bayyanar a cikin ME ME Kungiyar da ta nuna babu wani bambanci mai mahimmanci (p> 0.05).
An yi bincike na BLOL blot da ake yi amfani da kayan aikin rigakafi da IL-4 da IL-17 don tabbatar da tsarin bayyanar Mrna. Kamar yadda aka nuna a cikin Figures 5a, B, idan aka kwatanta da ni kungiyar da kuma wasan kwaikwayon na Sham, matakan samar da Il-4 da IC-17 a cikin Npeungiyar Npe. Idan aka kwatanta da kungiyar ayyukan Sham, matakan sunadari na il-4 da IL-17 a cikin Mayu mahimman canje-canje (p> 0.05).
(A) matakan furotin na IL-4 da IL-17 a cikin kungiyar Npe sun fi wadanda ke cikin kungiyar da ke cikin kungiyar da kuma Kungiyar Placebo (P <005). (B) Scotogram na yamma.
Saboda iyakataccen adadin samfuran mutane da aka samu lokacin tiyata, a bayyane kuma cikakkun karatun karatu akan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cututtukan cuta na MC suna da wahala. Munyi kokarin kafa tsarin dabba na MC don yin nazarin hanyoyin ta. A lokaci guda, kimantawa kimantawa, kimiyyar hatsantarwa da kimantawa na ilimin halittar motsa jiki ana amfani da kimanta hanya na MC ya jawo NP AutoRograft. A sakamakon haka, samfurin NP ya haifar da canjin hankali a cikin sati 12 zuwa maki na mako-mako a cikin T2wal a cikin T2wal a cikin T2wal a cikin T2wal a cikin T2wal a cikin T2wal a cikin T2w Subs), yana daɗaɗa canje-canje, da kwayoyin halitta da kwayoyin halitta kimantawa nazarin halittu ya tabbatar da sakamakon binciken radiological.
Sakamakon wannan gwajin yana nuna cewa canje-canjen tarihi da ilimin tarihi suka faru a wurin ƙeta na ƙwaƙwalwa na vertebral a cikin NPEA. A lokaci guda, bayyanar il-4, IL-17 da IFN-γ-17 da Ilyn-γ, IF-17 da Ilyn-γ, IF-17 da IFN-γ, IF-17 da IFN-γ 4 and jiki na iya haifar da jerin siginar da cututtukan cututtuka. Abu ne mai sauki ka ga cewa halayen sigogi na kayan dabbobi (low sigina a cikin jerin T1w a cikin jerin sel na T2w) sun yi kama da na sel na T2w) sun yi kama da na sel na T2w Tabbatar da lura da ilimin gurguzi da babban dabbar, ita ce, canje-canje a cikin sel na tarko suna ci gaba. Kodayake amsar kumburi ta haifar da rauni m rauni na iya bayyana nan da nan bayan huda, MRI ya nuna cewa a sati 20 ba tare da wasu alamun murmurewa ba ko kuma sake fasalin musayar Mri. Wadannan sakamakon suna nuna cewa autologous verterebral NP shine abin dogara hanya don kafa ci gaba mv a cikin zomaye.
Wannan samfurin na hangen ne yana buƙatar isasshen fasaha, lokaci, da ƙoƙarin tiyata. A cikin gwaje-gwajen farko, dissectection ko wuce gona da iri na jijiyoyin zuciya na gari na iya haifar da samuwar osteofhyes. Ya kamata a kula kada ya lalace ko haushi da fayel na kusa. Tun da zurfin shigar azzakari cikin rufi dole ne a sarrafa shi don samun sakamako mai daidaituwa da haifuwa, mun ɗora hannu ta yankan sheath na tsawon 3 mm. Yin amfani da wannan zane yana tabbatar da zurfin hayaki a cikin jikin vertebal. A cikin gwaje-gwajen farko, likitoci uku na Orthopedic sun shiga cikin aikin da aka samo a cikin allurai na 18-ma'auni don aiki tare da buƙatun 18-ma'aunin. Don kauce wa matsanancin zubar da jini yayin hakowar, har yanzu yana da allura har yanzu na ɗan lokaci zai samar da ƙarin rami mai dacewa, yana ba da shawara cewa an sarrafa takamaiman matakin MC ta wannan hanyar.
Ko da yake karatun da yawa sun yi niyya ga MC, kadan sananne ne game da Etiology da Pathogenesis na MC25,26,27. Dangane da karatunmu na baya, mun gano cewa autoimmunnations yana taka rawa sosai a cikin abin da ya faru da ci gaban MC12. Wannan binciken ya yi nazari game da magana mai yawa na IL-4, I IFN-γ, waɗanda sune manyan hanyoyin bambance-bambancen ƙwayoyin CD4 + bayan ƙwayoyin cuta. A cikin binciken mu, idan aka kwatanta da mummunan rukunin, rukunin NPE yana da cikakkiyar magana ta IL-4, I Ilyn-γ 4 da IN INNA sun yi sama.
Aure, Il-17 magana mrna tana ƙaruwa a cikin sel na NP daga marasa lafiya tare da diski herniation28. Har ila yau, an sami matakan Il-4 da γ-γ mara ma'ana wanda rashin daidaituwa na rashin daidaituwa na ƙiyayya mara amfani idan aka kwatanta da ikon kula da lafiya29. IL-17 taka muhimmiyar rawa a cikin kumburi, raunin nama a cikin cututtukan autoimms3030 kuma yana haɓaka amsar rigakafi ga IFN-γ31. An bayar da rahoton rauni na IL-17-Appeed. IL-4 na iya hana bayyana yanayin Cytiyanines (kamar Il-1β da TNFα) da kuma dawowa na Macrophage34. An ruwaito cewa bayyanar Mrna ta IL-4 ya banbanta a cikin npe kungiyar idan aka kwatanta da IL-17 da IFN-γ a lokaci guda; Bayanin Mrna na Ifn-γ a cikin rukunin NPE ya fi wannan muhimmanci a sauran kungiyoyin. Saboda haka, IFN-γ γ Overormation na iya zama matsakanci na kumburi amsawar da aka jawo ta NP ta hanyar amfani da NP. Nazari ya nuna cewa INN-γ γ
Wannan binciken ya ba da shawarar cewa amsawar ta atomatik na iya kasancewa cikin abin da ya faru da ci gaban MC. Luoma et al. Mun gano cewa halayen alamun MC da manyan NP suna kama da MRI, kuma duka suna nuna alamar alama a T2w jerin38. Wasu an tabbatar da cewa an tabbatar da cewa Cyttoines suna da alaƙa da abin da ya faru na MC, kamar Il-139. Mah et al. An ba da shawarar cewa sama ko ƙasa da ƙasa na NP na iya samun babban tasiri a kan abin da ya faru da ci gaban MC12. Bobko40 da Herzbein et al.41 sun ba da rahoton cewa NP ne ɓataccen nama wanda ba zai iya shiga cikin kewaya ba daga haihuwa. NP Enribusions gabatar da jikin kasashen waje a cikin samar da jinin, ta hanyar samar da kayan aikin samar da jini42. Abubuwan da ke cikin Autoimmins na iya haifar da yawancin dalilai masu rigakafi, kuma lokacin da waɗannan abubuwan ana ci gaba da fallasa kyallen takarda, za su iya haifar da canje-canje a cikin siginar -33. A cikin wannan binciken, abubuwan da ke faruwa na IL-4, IF-17 da IFN-γ 6,000 ne dalilai na kwarai, kara tabbatar da kusanci tsakanin NP da MCS44. Wannan samfurin dabba da kyau mimics da NP basewa da shigarwa cikin ƙarshen farantin. Wannan tsari ya kara bayyana tasirin autoimmonity a kan MC.
Kamar yadda ake tsammani, wannan samfurin dabba yana samar mana da yiwuwar dandamali don nazarin MC. Koyaya, wannan ƙirar har yanzu tana da wasu iyakoki: Da fari dai, yayin sauraron dabbobi, wasu dabbobi masu gwajin tarihi, don haka wasu dabbobi "suka faɗi daga amfani" akan lokaci. Abu na biyu, kodayake ana saita maki lokaci guda uku a cikin wannan binciken, muna iya daidaita nau'in MC (nau'in modic ɗin da nake canzawa), don haka bai isa ya wakiltar aiwatar da aikin ci ba mafi kyawun lura da duk canje-canje na siginar. Abu na uku, canje-canje a cikin tsarin nama ana iya bayyana ta hanyar lalata tarihin tarihi, amma wasu dabaru na musamman zasu iya bayyana canje-canje na Microstractalididdiga a cikin wannan ƙirar. Misali, an yi amfani da micrerizy mai haske Microscopy don bincika samuwar Fibrongt na FIBGBST45. Tasirin Tasirin NP na NP akan MC da kuma ƙarshen buƙatar ƙarin nazari.
HISTTY da hudu maza na New Zealand Farin zomaye (nauyi game da 2.5-3 kilogiram) ya kasu da yawa zuwa kungiyar ta Sham Groups (min rukuni) da kungiyar NPEVE). Kwamitin gwaji na Tianjin Asibiti ya amince da shi, kuma hanyoyin gwaji da za'ayi ne suka aiwatar da su sosai.
An yi wasu cigaba ga dabarun tiyata na S. Sobajima 46. Kowane zomo an sanya shi a cikin matsayin mai ɗaukar hoto da kuma yanayin rashin daidaituwa na diski guda biyar a jere (IVDs) ya fallasa ta amfani da tsarin tafiyar hawainar ra'ayi. Kowane zomo ya ba da maganin maganin sa maye (20% urethane, 5 ml / kg ta jijiyoyin kunne). An sanya fata mai dadadi daga ƙananan gefen haƙarƙarin zuwa ƙwararren pelvic Bermal, 2 cm ventral zuwa tsokoki na parverebral. An fallasa kashin baya na dama daga L1 zuwa L6 Sharp ya fallasa ta hanyar tsinkaye na mamaye nama, da tsokoki (fig. 6A). An ƙaddara matakin diski ta amfani da ƙwararren pelvic a matsayin alamar yanayin yanayin yanayin l5-L6. Yi amfani da allura a kan 2-grature don rawar soja don rawar soja a kusa da farantin ƙarshen l5 vertebra zuwa zurfin 3 mm (Fig. 6b). Yi amfani da sirinji na ml ml don tallafawa yanayin yanayin motsa jiki a cikin diski na diski a cikin diski na L1-l2 (Fig .c). Cire nucleus pulposus ko tsoka bisa ga bukatun kowane rukuni. Bayan ramin rawar soja, an sanya daskararre square a kan zurfin fascia da fata, da kulawa kada su lalata ƙwayar ƙwayar jijiyoyin jiki a cikin tiyata.
(A) a) L5-L6 dis Disc ya fallasa ta hanyar tsarin rasani na koma baya. (B) Yi amfani da allura 16-gemu don rawar soja wani rami kusa da l5 ƙare. (C) MFS MFS an girbe.
Janar Innesthea da aka gudanar da kashi 20% Urethane (5 ml / kg) an maimaita ta hanyar jijiyoyin kunne, da makonni 20, da makonni 20.
An sadaukar da zomaye ta hanyar allurar kututture (25.0 MG / kg) da sodium na pentobarbital (1.2 G / kg) a 12, makonni 20 bayan tiyata. An cire dukkan kashin baya don bincike na tarihi da nazarin na ainihi. Anyi amfani da tresscit na baya mai yawa (RT-QPCR) da kuma ana amfani da birgewa na yamma don gano canje-canje a cikin abubuwan da ke cikin rigakafi.
An yi masu binciken MRR a zomaye ta amfani da Clinical na 70 t THE magnet (FLorence, sc) sanye take da reces mai karba. An sanya zomaye tare da 20% Urethane (5 ml / kg) ta jijiyoyin kunne sannan kuma a sanya su superine coil (Ge Media tsarin). Hotunan Balaguro na Joronal T2-We, 1445 ms; Te, 37 ms) an sami damar ayyana wurin da lumbar diski Disc daga L3-L4 zuwa L5-L6. An samo yanka na sagittal na Sagittal da kebules tare da saitunan masu zuwa: Sigin-eCho jerin tare da maimaitawa (tr) lokacin 2200 (Te) na 70 MS, Matrix; filin gani na 260 da takwas motsin motsa jiki; Kauri kauri ya kasance 2 mm, rata yana 0.2 mm.
Bayan an dauki hoto na ƙarshe kuma aka kashe na ƙarshe na ƙarshe, Sham, tsoka-exbedded, kuma an cire np fayafai don jarrabawar ta tarihi. An daidaita kyallen takarda a cikin 100% na tsaka tsaki da tsari na mako 1, da aka lalata da ethylenediaminettattaetttttttttttttttatta acid, da paraffin seconed. An saka shinge na nama a cikin paraffin kuma a yanka a sassan sagittal (5 μm lokacin farin ciki) ta amfani da microtome. An lalata sassan da hematoxylin da Eosin (H & E).
Bayan tattara bayanan da ke tsakaninsu daga zomaye a cikin kowane rukuni, an fitar da Timple (Shanghai Sangon Tsarin masana'antar da improm II na Transrit (Promeaga Inc. , Madison, Wi, Amurka). An yi watsi da rubutun
An yi amfani da RT-QPCR ta amfani da m 7300 (kayan tarihin Biosystemems Inc., Amurka) da Sigma-Aldrich (Sigma-Aldrich (Sigma-Aldrich (Sigma-Aldrich (Sigma-Aldrich (Sigma-Aldrich (Sigma-Aldrich (Sigma-Aldrich, St. Louis, Mouis, Mouis, Mouis, Mouis, Mouis, Mouis, Mouis, Mouis, Mouis, Mouis, Mouis. Vecr dauki girma shine 20 μl kuma dauke da 1.5 μl na diluted CDNA da 0.2 μm kowane ɗayan. Maƙerin Oligoperight sun tsara firayimurrime (gayyatar Valencia, CA) da kuma keran Nanjing Golden Stewart Beanechnology Co., Ltd. (China 1). An yi amfani da yanayin cinikin da ke tattare da wannan yanayin: fara kunna aikin polymes a 94 ° C na 15 min a 64 ° C, karin bayani. An yi ma'aunai na tsawon min 5 a 72 ° C. Duk samfuran an samar da su sau uku kuma ana amfani da darajar matsakaita don bincike na RT-QPCR. An bincika bayanan amplification ta amfani da flextation 3 (na'urorin kwayoyin, Sunnyvale, CA, Amurka). IL-4, IL-17, kuma IFN-γ Gane magana da aka daidaita zuwa ga ikon sarrafawa (ActB). An ƙididdige matakan bayyanar Mrna ta amfani da hanyar 2-δδect.
Jimlar furotin da aka samo daga kwai ta amfani da homogenish a ripshante da kuma cockphatase a cikin 20 min at 4 ° C don cire tarkace nama. Microst micros na furotin an ɗora shi a cikin layi, rabu da kashi 10% SDS-shafi, sannan ya canza zuwa membrane pvdf. An yi katangar da aka yi a cikin 5% mara bushe madara a cikin trish-bufeded saline (tbs) wanda ya ƙunshi 0.1% tween 20 don 1 h a ɗakin zafin jiki. An sanya membrane da anti-anti-degit na zomo-ecoros na farko (diluted 1: 200; Boster, Wuhan, 200; Boss, na dare a 4 ° C kuma China) a karo na biyu; Tare da sakandare na sakandare (Goat antioglobulin g at 1: 40,000 diliyya) hade tare da horseradi) peremosh peroxidase (Boster, Wuhan, awa china a dakin da zazzabi. An gano sigina na yamma ta hanyar ƙara chemilumananscence na a kan membrane na chemilumincent bayan iska mai iska. Don bincike na Denitometric na Denaitomer, blots an bincika kuma an ƙididdige ta amfani da software na ban kasuwa kuma sakamakon ya bayyana a matsayin abin da ya yi niyya ga rigakafi ga rashin kariya.
An yi lissafin ƙididdiga ta amfani da kunshin software ɗin spess16 (SPSS, Amurka). An tattara bayanan yayin binciken a matsayin ma'anar ± SD) kuma an bincika ± SD) da kuma bincika matakan bincike game da bambance-bambancen ra'ayi (Anova) don ƙayyade bambance-bambance tsakanin rukunoni biyu. P <0.05 an dauke shi da muhimmanci sosai.
Don haka, kafuwar samfurin dabba na MC ta hanyar dasa assologros NPS a cikin jikin macroanatom, ƙididdigar ilimin tarihin Macroanatomic, ƙididdigar ilimin tarihin Macroanatomic, ƙididdigar ilimin tarihin Macroanatomic, ƙididdigar ilimin tarihi ayyukan shiga.
Yadda za a buga wannan labarin: Han, C. et al. An kafa tsarin dabba na canje-canje na dabba da keɓance autous nucleus cikin ƙashin ƙashin lumbar. Sci. Rep. 6, 35102: 10.1038 / srep35102 (2016).
Weishupt, D., Zanetti, M., Todler, J., da kuma boos, na Tasirin distormits, da kuma farantin hadin gwiwa, da facet hadin gwiwa hadin kai a cikin asypmpomatic . kudi. Radiology 209, 661-666, doi: 10.1148 / Radiology.2209.3844656 (1998).
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De Roos, A., cringzer, K., da kuma Dalinka, M. MRR na Macewar ƙashi na ƙashi a cikin cutar ƙarshen hanyar lumbar kashin baya. Ajr. Jaridar Amurka ta Radiology 149, 531-534, Doi: 10.2214 / AJR.149.3.531 (1987).
Modic, MT, Steinberg, PM, Ross, JS, Masaryk, TJ Daraja, Ka'idar Marina na JR Dogara tare da Mri. Radiology 166, 193-199, Doi: 10.1148 / Radiology.166.1.333366678 (1988).
Modic, MT, Masaryk, TJ, Ross, JS, da Carter, JR yana tunanin cutar da Disc Cutar cuta cuta. Radiology 168, 177-186, doi: 10.1148 / Radiology.168.1.32.3289089 (1988).
Jensen, ts, et al. Masu annabta na neovetherbral kafin canje-canje na siginar a cikin yawan jama'a. Jaridar Turai ta Turai: Jama'ar Jam'iyyar Turai ta Turai, ta Turai ta Turai ta Turai ta Turai, da 'yan Turai, 129-135, S00586-009-1184-5 (2010).
Albert, HB da Mannansch, K. Modic Canja bayan Lumbar Disc Herniation. Jaridar Turai ta Turai: Jama'ar Jam'iyyar Turai ta Turai, kungiyar Turai ta Turai ta Turai ta Turai ta Cervical Pine ta 16, 977-0982, S00586-007-0136-8 (2007).
Kertulu, L., Modelas, T., Gronblad, Nau'in Modic i Musanya da sauri. Do Diecia 21, 1135-1142, Doi: 10.1007 / s00586-012-2147-9 (2012).
Hu, ZJ, Zhao, FD, Fang, XQ da fan, sw modic canje-canje: mai yiwuwa yana haifar da gudummawa ga lumbar disc yanke. Annuwa na likita 73, 930-932, Doi: 10.1016 / j.MEY.2 (2009) (2009).
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